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ArcticZymes – Cool Heat-Labile Enzymes for Molecular Biology

ArcticZymes develops and markets a range of specialised cold adapted enzymes for molecular biology applications. From the location in Tromsø, far North in Norway ArcticZymes is an active participant in a number of bioprospecting programs in the cold Arctic Sea, aiming at developing new enzymes for molecular biology.

Unique cold adapted enzymes making big problems simple

Typically associated with cold activity is an inherent heat-lability that causes ArcticZymes enzymes to be irreversibly heat-inactivated by moderate heat treatment. This feature is essential in applications where you want to use a number of enzymes in sequence, and at the same time the heat-lability offers a simple workflow as opposed to extraction of the enzyme which would be the alternative.This is a costly, time consuming method with substantial loss of sample as a consequence.

Simple workflows using heat-labile enzymes

ArcticZymes today markets 5 different enzymes, all with unique characteristics that are setting the market standard and all are recombinantly manufactured.

Shrimp Alkaline Phosphatase (SAP) from Arctic Shrimp, has been on the market since 1993 and was the first heat-labile alkaline phosphatase as opposed to Calf Intestinal Alkaline Phosphatase. SAP is now the market standard for heat-labile phosphatases, and can now be obtained directly from ArcticZymes after an exclusive contract with a partner ended.

Cod Uracil-DNA Glycosylase (Cod UNG, UDG) is the only UNG (UDG) on the market that is irreversibly heat-inactivated. Therefore you should use this enzyme for carry-over protection whenever you want to do post-PCR operations with your PCR fragment.

Cod UNG

dsDNase/HL-dsDNase wildtype and mutant of a double-strand specific DNase from Arctic Shrimp. dsDNase inactivates at 65°C whereas the more heat-labile mutant inactivates at 55°C. The double strand specificity allows for cleaning RNA samples from gDNA contamination, and cleaning PCR mastermixes from gDNA contamination (typically originating from Taq Polymerase made in E. coli) while preserving single stranded DNA molecules like primers and probes.

Thus contamination control until the moment the template is added can be offered. In the context of RNA templates the template can also be added to the reaction before the HL-dsDNase is inactivated. The HL-dsDNase inactivation temperature is low enough to be very gentle to small RNA species and retains these RNAs in the sample much better than column techniques.

Salt Active Nuclease is a general nuclease with activity optimum at 0,5 M NaCl that allows for nuclease digestion in standard protein buffers. The high salinity allows for more complete digestion of contaminating RNA/DNA as the affinity of RNA/DNA to strand-attached proteins is looser. This enzyme is not heat-labile as such, but a patent for a more heat-labile version has been filed. Launch of that enzyme is scheduled for second half year 2012.

image 1

Heat&Run™ is a kit for removal of DNA contamination in RNA preparations prior to PCR. The kit offers a simple workflow - a short digestion with the HL-dsDNase, followed by heat inactivation - and ready to "Run"! No column chromatography, no phenol/chloroform and no loss of sample due to extraction. The kit will be launched 1 August 2012.

Normalization Kit is a kit for normalizing libraries prior to sequencing - enriching for rare transcripts, and reducing the occupation of sequencing capacity by abundant sequences. The kit is due for launch in the Autumn of 2012, and will offer a substantially simpler workflow than current marketed alternatives yet delivering superior performance.

Better reproducibility and sensitivity without loss of sample

With their active participation in new bioprospecting ArcticZymes is working on launching a range of radically new enzymes that is due to set new standards in the field of molecular biology. Combining the excellent processivity of the cold active enzymes with heat-lability will allow for simple and fast workflows for the benefit of science.

Tromso

Contact

ArcticZymes AS
Sykehusveien 23
NO-9019 Tromsø
Norway

Postal address:
ArcticZymes AS
P.O. Box 6463
NO-9294 Tromsø
Norway

Tel: +47 77 64 89 00
E-mail addresses:
orders@arcticzymes.com - for placing orders
support@arcticzymes.com - for technical inquiries
contact@arcticzymes.com - for general inquiries

URL: www.arcticzymes.com